diff --git a/drivers/spi/Kconfig.nrfx b/drivers/spi/Kconfig.nrfx
index f3afa79c4c3..e609a6c4933 100644
--- a/drivers/spi/Kconfig.nrfx
+++ b/drivers/spi/Kconfig.nrfx
@@ -28,6 +28,7 @@ config SPI_NRFX_SPIM
 	select NRFX_SPIM3 if HAS_HW_NRF_SPIM3
 	select NRFX_SPIM4 if HAS_HW_NRF_SPIM4
 	select NRFX_SPIM00 if HAS_HW_NRF_SPIM00
+	select NRFX_SPIM01 if HAS_HW_NRF_SPIM01
 	select NRFX_SPIM20 if HAS_HW_NRF_SPIM20
 	select NRFX_SPIM21 if HAS_HW_NRF_SPIM21
 	select NRFX_SPIM22 if HAS_HW_NRF_SPIM22
@@ -54,6 +55,7 @@ config SPI_NRFX_SPIS
 	select NRFX_SPIS2 if HAS_HW_NRF_SPIS2
 	select NRFX_SPIS3 if HAS_HW_NRF_SPIS3
 	select NRFX_SPIS00 if HAS_HW_NRF_SPIS00
+	select NRFX_SPIS01 if HAS_HW_NRF_SPIS01
 	select NRFX_SPIS20 if HAS_HW_NRF_SPIS20
 	select NRFX_SPIS21 if HAS_HW_NRF_SPIS21
 	select NRFX_SPIS22 if HAS_HW_NRF_SPIS22
diff --git a/modules/hal_nordic/nrfx/Kconfig b/modules/hal_nordic/nrfx/Kconfig
index 80610599e60..be746188faa 100644
--- a/modules/hal_nordic/nrfx/Kconfig
+++ b/modules/hal_nordic/nrfx/Kconfig
@@ -547,6 +547,11 @@ config NRFX_SPIM00
 	depends on $(dt_nodelabel_exists,spi00)
 	select NRFX_SPIM
 
+config NRFX_SPIM01
+	bool "SPIM01 driver instance"
+	depends on $(dt_nodelabel_exists,spi01)
+	select NRFX_SPIM
+
 config NRFX_SPIM20
 	bool "SPIM20 driver instance"
 	depends on $(dt_nodelabel_exists,spi20)
@@ -655,6 +660,11 @@ config NRFX_SPIS00
 	depends on $(dt_nodelabel_exists,spi00)
 	select NRFX_SPIS
 
+config NRFX_SPIS01
+	bool "SPIS01 driver instance"
+	depends on $(dt_nodelabel_exists,spi01)
+	select NRFX_SPIS
+
 config NRFX_SPIS20
 	bool "SPIS20 driver instance"
 	depends on $(dt_nodelabel_exists,spi20)
diff --git a/modules/hal_nordic/nrfx/nrfx_kconfig.h b/modules/hal_nordic/nrfx/nrfx_kconfig.h
index 191855b3ff0..36a09308131 100644
--- a/modules/hal_nordic/nrfx/nrfx_kconfig.h
+++ b/modules/hal_nordic/nrfx/nrfx_kconfig.h
@@ -503,6 +503,9 @@
 #ifdef CONFIG_NRFX_SPIM00
 #define NRFX_SPIM00_ENABLED 1
 #endif
+#ifdef CONFIG_NRFX_SPIM01
+#define NRFX_SPIM01_ENABLED 1
+#endif
 #ifdef CONFIG_NRFX_SPIM20
 #define NRFX_SPIM20_ENABLED 1
 #endif
@@ -573,6 +576,9 @@
 #ifdef CONFIG_NRFX_SPIS00
 #define NRFX_SPIS00_ENABLED 1
 #endif
+#ifdef CONFIG_NRFX_SPIS01
+#define NRFX_SPIS01_ENABLED 1
+#endif
 #ifdef CONFIG_NRFX_SPIS20
 #define NRFX_SPIS20_ENABLED 1
 #endif
diff --git a/soc/nordic/common/Kconfig.peripherals b/soc/nordic/common/Kconfig.peripherals
index 083fcc4e089..d7e489b446a 100644
--- a/soc/nordic/common/Kconfig.peripherals
+++ b/soc/nordic/common/Kconfig.peripherals
@@ -271,6 +271,9 @@ config HAS_HW_NRF_SPIM4
 config HAS_HW_NRF_SPIM00
 	def_bool $(dt_nodelabel_enabled_with_compat,spi00,$(DT_COMPAT_NORDIC_NRF_SPIM))
 
+config HAS_HW_NRF_SPIM01
+	def_bool $(dt_nodelabel_enabled_with_compat,spi01,$(DT_COMPAT_NORDIC_NRF_SPIM))
+
 config HAS_HW_NRF_SPIM20
 	def_bool $(dt_nodelabel_enabled_with_compat,spi20,$(DT_COMPAT_NORDIC_NRF_SPIM))
 
@@ -334,6 +337,9 @@ config HAS_HW_NRF_SPIS3
 config HAS_HW_NRF_SPIS00
 	def_bool $(dt_nodelabel_enabled_with_compat,spi00,$(DT_COMPAT_NORDIC_NRF_SPIS))
 
+config HAS_HW_NRF_SPIS01
+	def_bool $(dt_nodelabel_enabled_with_compat,spi01,$(DT_COMPAT_NORDIC_NRF_SPIS))
+
 config HAS_HW_NRF_SPIS20
 	def_bool $(dt_nodelabel_enabled_with_compat,spi20,$(DT_COMPAT_NORDIC_NRF_SPIS))
 
diff --git a/soc/nordic/validate_base_addresses.c b/soc/nordic/validate_base_addresses.c
index 1c5c39f74ec..e065aa7ccff 100644
--- a/soc/nordic/validate_base_addresses.c
+++ b/soc/nordic/validate_base_addresses.c
@@ -265,6 +265,7 @@ CHECK_SPI_REG(spi2, 2);
 CHECK_DT_REG(spi3, NRF_SPIM3);
 CHECK_DT_REG(spi4, NRF_SPIM4);
 CHECK_DT_REG(spi00, NRF_SPIM00);
+CHECK_DT_REG(spi01, NRF_SPIM01);
 CHECK_DT_REG(spi20, NRF_SPIM20);
 CHECK_DT_REG(spi21, NRF_SPIM21);
 CHECK_DT_REG(spi22, NRF_SPIM22);