diff --git a/tests/bsim/bluetooth/audio/overlay-bt_ll_sw_split.conf b/tests/bsim/bluetooth/audio/overlay-bt_ll_sw_split.conf
index cf40449c4cc..a8dcadfbca6 100644
--- a/tests/bsim/bluetooth/audio/overlay-bt_ll_sw_split.conf
+++ b/tests/bsim/bluetooth/audio/overlay-bt_ll_sw_split.conf
@@ -51,8 +51,8 @@ CONFIG_BT_CTLR_ADV_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_SENDER=y
 CONFIG_BT_CTLR_ADV_ISO=y
 CONFIG_BT_CTLR_ADV_ISO_SET=1
-CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=2
-CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
+CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=4
 CONFIG_BT_CTLR_ADV_ISO_PDU_LEN_MAX=247
 
 # ISO Receive Controller
@@ -61,8 +61,8 @@ CONFIG_BT_CTLR_SYNC_PERIODIC=y
 CONFIG_BT_CTLR_SYNC_TRANSFER_RECEIVER=y
 CONFIG_BT_CTLR_SYNC_ISO=y
 CONFIG_BT_CTLR_SCAN_SYNC_ISO_SET=1
-CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=2
-CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=2
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=4
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=4
 CONFIG_BT_CTLR_SYNC_ISO_PDU_LEN_MAX=251
 
 # ISO Connection Oriented
@@ -76,12 +76,12 @@ CONFIG_BT_CTLR_CONN_ISO_PDU_LEN_MAX=251
 CONFIG_BT_CTLR_CONN_ISO_LOW_LATENCY_POLICY=y
 
 # ISO Transmissions
-CONFIG_BT_CTLR_ISOAL_SOURCES=2
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
 CONFIG_BT_CTLR_ISO_TX_BUFFERS=18
 CONFIG_BT_CTLR_ISO_TX_BUFFER_SIZE=255
 
 # ISO Receptions
-CONFIG_BT_CTLR_ISOAL_SINKS=2
+CONFIG_BT_CTLR_ISOAL_SINKS=4
 CONFIG_BT_CTLR_ISO_RX_BUFFERS=8
 
 # Tx Power Dynamic Control
diff --git a/tests/bsim/bluetooth/audio/overlay-nrf5340_cpunet_iso-bt_ll_sw_split.conf b/tests/bsim/bluetooth/audio/overlay-nrf5340_cpunet_iso-bt_ll_sw_split.conf
index 4e61f830892..d90eca55e7c 100644
--- a/tests/bsim/bluetooth/audio/overlay-nrf5340_cpunet_iso-bt_ll_sw_split.conf
+++ b/tests/bsim/bluetooth/audio/overlay-nrf5340_cpunet_iso-bt_ll_sw_split.conf
@@ -5,3 +5,12 @@
 CONFIG_BT_ISO_TX_BUF_COUNT=18
 
 CONFIG_BT_CTLR_ISO_TX_BUFFERS=18
+
+CONFIG_BT_CTLR_ADV_ISO_STREAM_COUNT=4
+CONFIG_BT_CTLR_ADV_ISO_STREAM_MAX=4
+
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_COUNT=4
+CONFIG_BT_CTLR_SYNC_ISO_STREAM_MAX=4
+
+CONFIG_BT_CTLR_ISOAL_SOURCES=4
+CONFIG_BT_CTLR_ISOAL_SINKS=4
diff --git a/tests/bsim/bluetooth/audio/prj.conf b/tests/bsim/bluetooth/audio/prj.conf
index 8c88146875a..dcc7fd5f40f 100644
--- a/tests/bsim/bluetooth/audio/prj.conf
+++ b/tests/bsim/bluetooth/audio/prj.conf
@@ -44,10 +44,10 @@ CONFIG_BT_BAP_BROADCAST_SOURCE=y
 CONFIG_BT_BAP_BROADCAST_SINK=y
 CONFIG_BT_AUDIO_CODEC_CFG_MAX_METADATA_SIZE=196
 CONFIG_BT_AUDIO_CODEC_CAP_MAX_METADATA_SIZE=196
-CONFIG_BT_BAP_BROADCAST_SRC_STREAM_COUNT=2
-CONFIG_BT_BAP_BROADCAST_SRC_SUBGROUP_COUNT=1
-CONFIG_BT_BAP_BROADCAST_SNK_SUBGROUP_COUNT=1
-CONFIG_BT_BAP_BROADCAST_SNK_STREAM_COUNT=2
+CONFIG_BT_BAP_BROADCAST_SRC_STREAM_COUNT=4
+CONFIG_BT_BAP_BROADCAST_SRC_SUBGROUP_COUNT=2
+CONFIG_BT_BAP_BROADCAST_SNK_SUBGROUP_COUNT=2
+CONFIG_BT_BAP_BROADCAST_SNK_STREAM_COUNT=4
 CONFIG_BT_ISO_PERIPHERAL=y
 CONFIG_BT_ISO_MAX_CHAN=4
 CONFIG_BT_ISO_TX_MTU=310
@@ -131,7 +131,7 @@ CONFIG_BT_OTS_CLIENT=y
 
 # Broadcast Audio Scan Service and client
 CONFIG_BT_BAP_SCAN_DELEGATOR=y
-CONFIG_BT_BAP_BASS_MAX_SUBGROUPS=1
+CONFIG_BT_BAP_BASS_MAX_SUBGROUPS=2
 CONFIG_BT_AUDIO_CODEC_CFG_MAX_METADATA_SIZE=255
 CONFIG_BT_BAP_BROADCAST_ASSISTANT=y
 
diff --git a/tests/bsim/bluetooth/audio/test_scripts/_bap_broadcast.sh b/tests/bsim/bluetooth/audio/test_scripts/_bap_broadcast.sh
new file mode 100755
index 00000000000..b31ddd057fe
--- /dev/null
+++ b/tests/bsim/bluetooth/audio/test_scripts/_bap_broadcast.sh
@@ -0,0 +1,17 @@
+#!/usr/bin/env bash
+#
+# Copyright (c) 2024 Nordic Semiconductor ASA
+#
+# SPDX-License-Identifier: Apache-2.0
+
+dir_path=$(dirname "$0")
+
+set -e # Exit on error
+
+# Run all bap_broadcast_audio* tests
+for file in "$dir_path"/bap_broadcast_audio*.sh; do
+    if [ -f "$file" ]; then
+        echo "Running $file"
+        $file
+    fi
+done
diff --git a/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_multiple_group_multiple_bis.sh b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_multiple_group_multiple_bis.sh
new file mode 100755
index 00000000000..391f5c146c8
--- /dev/null
+++ b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_multiple_group_multiple_bis.sh
@@ -0,0 +1,28 @@
+#!/usr/bin/env bash
+#
+# Copyright (c) 2024 Nordic Semiconductor ASA
+#
+# SPDX-License-Identifier: Apache-2.0
+
+VERBOSITY_LEVEL=2
+EXECUTE_TIMEOUT=120
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+cd ${BSIM_OUT_PATH}/bin
+
+SIMULATION_ID="bap_broadcast_audio_multiple_group_multiple_bis"
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=0 -testid=broadcast_source \
+  -RealEncryption=1 -rs=23 -D=2 -argstest subgroup_cnt 2 streams_per_subgroup_cnt 2
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=1 -testid=broadcast_sink \
+  -RealEncryption=1 -rs=27 -D=2
+
+# Simulation time should be larger than the WAIT_TIME in common.h
+Execute ./bs_2G4_phy_v1 -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} \
+  -D=2 -sim_length=60e6 $@
+
+wait_for_background_jobs
diff --git a/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_multiple_group_single_bis.sh b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_multiple_group_single_bis.sh
new file mode 100755
index 00000000000..544887be7a2
--- /dev/null
+++ b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_multiple_group_single_bis.sh
@@ -0,0 +1,28 @@
+#!/usr/bin/env bash
+#
+# Copyright (c) 2024 Nordic Semiconductor ASA
+#
+# SPDX-License-Identifier: Apache-2.0
+
+VERBOSITY_LEVEL=2
+EXECUTE_TIMEOUT=120
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+cd ${BSIM_OUT_PATH}/bin
+
+SIMULATION_ID="bap_broadcast_audio_multiple_group_single_bis"
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=0 -testid=broadcast_source \
+  -RealEncryption=1 -rs=23 -D=2 -argstest subgroup_cnt 2 streams_per_subgroup_cnt 1
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=1 -testid=broadcast_sink \
+  -RealEncryption=1 -rs=27 -D=2
+
+# Simulation time should be larger than the WAIT_TIME in common.h
+Execute ./bs_2G4_phy_v1 -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} \
+  -D=2 -sim_length=60e6 $@
+
+wait_for_background_jobs
diff --git a/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_single_group_multiple_bis.sh b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_single_group_multiple_bis.sh
new file mode 100755
index 00000000000..9d50f018122
--- /dev/null
+++ b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_single_group_multiple_bis.sh
@@ -0,0 +1,28 @@
+#!/usr/bin/env bash
+#
+# Copyright (c) 2024 Nordic Semiconductor ASA
+#
+# SPDX-License-Identifier: Apache-2.0
+
+VERBOSITY_LEVEL=2
+EXECUTE_TIMEOUT=120
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+cd ${BSIM_OUT_PATH}/bin
+
+SIMULATION_ID="bap_broadcast_audio_single_group_multiple_bis"
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=0 -testid=broadcast_source \
+  -RealEncryption=1 -rs=23 -D=2 -argstest subgroup_cnt 1 streams_per_subgroup_cnt 2
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=1 -testid=broadcast_sink \
+  -RealEncryption=1 -rs=27 -D=2
+
+# Simulation time should be larger than the WAIT_TIME in common.h
+Execute ./bs_2G4_phy_v1 -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} \
+  -D=2 -sim_length=60e6 $@
+
+wait_for_background_jobs
diff --git a/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_single_group_single_bis.sh b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_single_group_single_bis.sh
new file mode 100755
index 00000000000..87b11003ab1
--- /dev/null
+++ b/tests/bsim/bluetooth/audio/test_scripts/bap_broadcast_audio_single_group_single_bis.sh
@@ -0,0 +1,28 @@
+#!/usr/bin/env bash
+#
+# Copyright (c) 2024 Nordic Semiconductor ASA
+#
+# SPDX-License-Identifier: Apache-2.0
+
+VERBOSITY_LEVEL=2
+EXECUTE_TIMEOUT=120
+
+source ${ZEPHYR_BASE}/tests/bsim/sh_common.source
+
+cd ${BSIM_OUT_PATH}/bin
+
+SIMULATION_ID="bap_broadcast_audio_single_group_single_bis"
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=0 -testid=broadcast_source \
+  -RealEncryption=1 -rs=23 -D=2 -argstest subgroup_cnt 1 streams_per_subgroup_cnt 1
+
+Execute ./bs_${BOARD_TS}_tests_bsim_bluetooth_audio_prj_conf \
+  -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} -d=1 -testid=broadcast_sink \
+  -RealEncryption=1 -rs=27 -D=2
+
+# Simulation time should be larger than the WAIT_TIME in common.h
+Execute ./bs_2G4_phy_v1 -v=${VERBOSITY_LEVEL} -s=${SIMULATION_ID} \
+  -D=2 -sim_length=60e6 $@
+
+wait_for_background_jobs